Weekly Changes in Finger‐Tip Prick Blood Highly Unsaturated Fatty Acid (HUFA) Composition with Acute Fish oil Supplementation and Washout in Men and Women
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Bibliographic record
Abstract
Finger‐tip prick blood (FTPB) collection is a potential research tool for monitoring eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The fatty acid composition of weekly FTPB samples collected from males and females in response to 4 week fish oil supplement intervention (providing 3.2 g EPA and 1.6 g DHA daily) followed by an 8 week washout period were examined. The fatty acid composition of plasma, red blood cell (RBC), and whole blood (WB) specimens collected by venous puncture at weeks 0, 4, 8 and 12 were analyzed for comparison. Nine males (22.4 ± 1.2 yrs, mean ± SD) and seven females (22.1 ± 1.8 yrs) completed the study. The percentage of n‐3 HUFA in total HUFA (n‐3 HUFA score) at baseline were similar for all blood samples (approximately 21–24). The n‐3 HUFA score in FTPB samples increased from week 0 (21.5 ± 2.2) to week 4 (51.8 ± 3.7) and decreased by week 12 (27.0 ± 1.5) but remained higher than week 0 (p < 0.05). FTPB and WB n‐3 HUFA scores were similar across time. In plasma, the n‐3 HUFA score increased 2.8 fold from week 0 (20.8 ± 2.2) to week 4 (57.7 ± 6.4) but rapidly returned to baseline at week 12 (24.3 ± 2.8). The RBC n‐3 HUFA score increased 1.6 fold from week 0 (23.6 ± 2.3) to week 4 (37.7 ± 3.0), but remained relatively high at week 12 (31.7 ± 2.4). In conclusion, finger‐tip prick blood resembled venous puncture whole blood supporting further development of FTPB collection as a fatty acid research tool.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it