Formate: a key metabolite for the fetus and neonatal animal (827.5)
Bibliographic record
Abstract
One‐carbon metabolism, mediated by tetrahydrofolate (THF), is critical for nucleotide synthesis and for methylation reactions, including DNA methylation and the synthesis of small molecules such as creatine and phosphatidylcholine. In addition to one‐carbon groups carried by THF it is apparent that formate is also a key intermediate in one‐carbon metabolism. Produced within mitochondria, formate is released to the cytosol, and incorporated into 10‐formyl‐THF where it may be directly used for purine synthesis or converted to more reduced forms for thymidylate synthesis and for the methylation of homocysteine to methionine. We employed a GC/MS, isotope dilution assay to measure formate levels in situations of rapid growth and development. Plasma [formate] was ~40 uM in adult rats; however, levels of ~400 uM were evident in neonatal (2‐3 days old) rats, declining to adult levels at about 56 days. Plasma [formate] was ~10 uM in non‐pregnant ewes; this increases to ~40 uM during pregnancy. Much higher levels were found in plasma from fetal lambs and amniotic fluid (116‐121 days gestation). We suggest that these data point to a key role for formate in growing organisms, possibly in distributing one‐carbon groups to different organs. (Supported by CIHR, RDC). Grant Funding Source : CIHR, RDC
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.008 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".