RAPID DETECTION OF SWINE TRANSMISSIBLE GASTROENTERITIS VIRUS BY NESTED POLYMERASE CHAIN REACTION
Bibliographic record
Abstract
The aim of this study was to develop a nested PCR for the rapid detection of TGEv. The primers were designed from the highly conserved regions of several sequences of this virus included in the analysis. With the purpose of achieving a good technical specificity, sequences of porcine epidemic diarrhoea virus (PEDv) were included in the design. This virus belongs to the same viral family and it has a close genetic relationship with TGEv. Since both viruses produce the same clinical signs, it was very important to get primers which not amplify PEDv. With the external primers, the amplification of a fragment of the expected size (441 pb) in all the samples evaluated by RT-PCR was obtained, but there was a very low intensity. In the second round (nested PCR), with the internal primers, the amplification of a fragment of the expected size of 168 pb was shown, with a very good concentration. It has been considered that when using nested PCR, TGEv detection sensitivity increased in isolates with low viral concentration. (
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".