SEROLOGICAL AND MOLECULAR CHARACTERIZATION OF ISOLATES OF PLUM POX VIRUS STRAIN EL AMAR TO BETTER UNDERSTAND ITS DIVERSITY, EVOLUTION, AND UNIQUE GEOGRAPHICAL DISTRIBUTION
Bibliographic record
Abstract
Sixteen isolates of Plum pox virus (PPV) were collected during a survey in the Egyptian areas of Sinro and Apoxa (El Fayoum) and El Amar (Nile Delta). All isolates reacted with the universal PPV monoclonal antibody MAb 5B and were identified as PPV strain EA by phylogenetic analysis of the full-length sequence of the coat protein (CP) gene. This classification was confirmed by detection with the strain-specific MAb EA24, except for an isolate denoted APR 50. Detailed analysis of the CP amino acid sequence of the EA isolates and epitope mapping revealed that histidine at amino acid position 65 of the CP sequence is an essential component of the epitope required for MAb EA24 recognition. APR 50 has an arginine substitution at this position. Five EA serogroups were identified, serogroup I being the prevailing one with 10 of the 14 isolates characterized. Moderate serological and relatively high genetic diversity was observed among isolates of PPV-EA. The most variable isolate, APR 48, contained a deletion of 33 nucleotides at the 5’ terminus of the CP gene. The relatively high genetic diversity of PPV-EA suggests that it is not a recent introduction.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.004 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.007 |
| Science and technology studies | 0.001 | 0.006 |
| Scholarly communication | 0.001 | 0.010 |
| Open science | 0.002 | 0.003 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".