Molecular characterization of a <i>Stagonospora nodorum</i> lipase gene <i>LIP1</i>
Bibliographic record
Abstract
A triglyceride lipase gene ( LIP1 ) was cloned from Stagonospora nodorum , the causal agent of wheat glume blotch. LIP1 encodes a 561 amino acid preproprotein with a predicted N ‐terminal signal peptide. Its expression was up‐regulated during plant infection and in culture media supplemented with saturated fatty glycerides. The recombinant Lip1 protein possessed lipolytic activity against a broad range of lipid substrates. When applied to wheat leaves, recombinant Lip1 decreased the hydrophobicity of the leaf surface, probably by liquefaction of epicuticular wax. Pretreatment of wheat leaves with Lip1 decreased the rate of conidial adhesion from 69·5% to 22·7% and from 58·9% to 28·4% in two independent assays based on different protocols. LIP1 replacement strains showed decreased lipolytic activity on culture media relative to the wild‐type strain, and adhesion of the conidia to the wheat leaf surface was impaired in the gene replacement strains. In two experiments, adhesion rates were 54·3% and 41·6% in the LIP1 replacement strains, as opposed to 77·7% and 66·6%, respectively, in the wild‐type. Collectively, the data demonstrate that the secreted lipase Lip1 is important for the adhesion of S. nodorum infection to wheat leaves.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".