Preliminary Molecular Studies of the First Report of Burkholderia pseudomallei Isolation from Soil Collected in the Amapá State, in Northern Brazil
Bibliographic record
Abstract
<p class="1Body">The wide spectra of colonizing microorganism, likewise <em>Burkholderia pseudomallei</em>, has been found in different habitats, and presenting distinct activity, as exerting physiological functions among plants, or as a pathogen for man, animals and also as a phytopathogen. A common disease of men and animals caused by <em>B. pseudomallei</em>, melioidosis, is a severe morbidity that usually culminates in the host death. Soil samples from different areas in the Amapá state, in northern Brazil, were screened for environmental microorganisms to assess potential antimicrobial activity aiming at biotechnological applications. Among the prospected microorganisms, <em>B. pseudomallei</em> was isolated from high humidity soils, mangrove, which is rich in organic materials, produced by the diversified local flora and fauna. The isolated <em>B. pseudomallei</em> was identified by its biochemical profile and growth characteristics. Molecular confirmation of <em>B. pseudomallei</em> phenotypic identification was achieved by PCR amplification of the 16 S ribosomal DNA. The sequencing of amplified products confirmed that the Amapá sample, and two other isolates from human infections in Ceará state, northeast Brazil, were <em>B. pseudomallei</em>, and sequence alignement to the same specie, MSHR146 strain from Australia, and clone YN01 from uncultured <em>Burkholderia</em> sp., deposited in the GenBank, exhibited close phylogenetic relationship among them. Until now, there is no report of <em>B. pseudomallei</em> related disease among human and animal populations in the Amapá state, despite the finding of <em>B. pseudomallei </em>in it, in an area of water buffalo ranching and flowing small rivers utilized by human populations.</p>
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".