ELUCIDATING THE INTERACTIONS OF THE GOLGI ASSOCIATED RETROGRADE PROTEIN (GARP) COMPLEX IN ARABIDOPSIS THALIANA.
Bibliographic record
Abstract
Plants contain a diversified endomembrane system and transport within the network requires efficient and accurate vesicle transport. Tethering factors represent a group of loosely related proteins and protein complexes that act to physically link vesicles to the target membrane. UNH was previously identified as the VPS51 homolog of the Golgi-associated retrograde protein (GARP) complex, known to tether vesicles in endosome to trans-golgi network (TGN) retrograde transport. The unh cellular phenotype is similar to secretion/vacuole defects observed in yeast and metazoan GARP complex mutants suggesting a conserved function in plants. This study was designed to assess whether the Arabidopsis GARP complex members participate in similar protein interactions as found in other eukaryotes. All of the plant GARP subunits contain predicted N-terminal coiled coils, important for interaction in other organisms. We show through deletional mutagenesis that these predicted N-terminal coiled coils are required for UNH to interact with VPS52 in vivo via yeast two-hybrid.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.003 | 0.001 |
| Research integrity | 0.000 | 0.002 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".