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Record W196170372 · doi:10.1096/fasebj.21.5.a334

Glutathione (GSH) Synthesis in healthy Adult Males:Effects of varying Cysteine Intake.

2007· article· en· W196170372 on OpenAlexaff
Glenda Courtney‐Martin, Mahroukh Rafii, Linda Wykes, Ronald O. Ball, Paul B. Pencharz

Bibliographic record

VenueThe FASEB Journal · 2007
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicSulfur Compounds in Biology
Canadian institutionsUniversity of AlbertaMcGill UniversityHospital for Sick Children
Fundersnot available
KeywordsGlutathioneCysteineMethionineGlycineChemistryAmino acidCysteine metabolismThiolLimitingInternal medicineEndocrinologyBiochemistryBiologyMedicineEnzyme

Abstract

fetched live from OpenAlex

Cysteine is generally considered the rate limiting substrate for GSH synthesis. However, there are no studies which have looked at whether GSH synthesis responds to graded levels of cysteine intake. The goal of this study was to determine whether increasing cysteine intake with methionine set at the estimated average requirement (EAR) of 14mg/kg/d and a protein intake of 1g/kg/d will result in increased GSH synthesis. We hypothesized that as cysteine intake increases, GSH synthesis will increase up to a point at which further increases in cysteine will have no effect on GSH synthesis. Fractional (FSR) and absolute synthesis rates (ASR)of GSH were measured in erythrocyte of healthy adult males during an infusion of [ 15 N, 13 C 2 ]glycine. Each subject was studied at 5 levels of cysteine intake. Preliminary results for 2 subjects show that GSH synthesis increases with increasing cysteine intake up to 20mg/kg/d. However, at intakes beyond 20mg/kg GSH synthesis decreased back to baseline levels. Our results suggest that in healthy adults, once the total sulphur amino acid requirements are met for protein synthesis, the body's need for GSH synthesis is also met. Effect of Cysteine Intake on GSH Synthesis Rates. image

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.258
Teacher spread0.246 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2007
Admission routes1
Has abstractyes

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