Oxidation of methionine residues: the missing link between stress and signalling responses in plants
Bibliographic record
Abstract
In response to biotic and abiotic stresses, plants induce a complex array of pathways and protein phosphorylation cascades which generally lead to a response aimed at mitigating the particular insult. In many cases, H2O2 has been implicated as the signalling molecule, but, although progress has been made in assembling the downstream components of these signalling pathways, far less is known about the mechanism by which the signal is perceived. In this issue of the Biochemical Journal, Hardin et al. provide evidence for a plausible mechanism by which plants perceive H2O2. Evidence is presented for chemical oxidation of methionine residues by H2O2 at critical hydrophobic positions within the canonical motifs that define the phosphorylation sites of a number of enzymes, thus inhibiting binding of protein kinases. This process is reversible by MSR (methionine sulfoxide reductase) activity in vivo. Using synthetic peptides for a number of enzymes which are phosphorylated by families of protein kinases, including the CDPK (calcium-dependent protein kinase) and AMPK (AMP-activated protein kinase) families, coupled with in vivo studies of assimilatory plant nitrate reductase, the authors demonstrate that this mechanism regulates the ability of kinases to bind the target protein, directly linking oxidative signals to changes in protein phosphorylation. These results may have widespread implications for the perception of redox signalling in plants and animals.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".