Mass spectrometry analysis of HIV-1 envelop proteins
Bibliographic record
Abstract
For many countries including Russia the development of preventive HIV vaccine became one of the crucial problems in the national survivorship. The purpose of the present study was to use phage display reverse panning (RP) technique for obtaining HIV-1 envelop proteins for LC-MS analysis of their major variability. The naïve human ScFv antibody library expressed on the M13 surface was created using total RNA from lymphocytes of 20 HIV-1 subtype A infected Russian patients. Standard RT with random hexanucleotide oligos, cDNA matrix heavy and light repertoires PCR-amplification, ScFv genes PCR assembly and pCANTAB5E phagemid cloning were used. HIV-1-specific human ScFv libraries were selected after three rounds of biopanning with recombinant diagnostic and native HIV-1 peptides, HIV specificity was confirmed with ELISA and WB, recombinant phages were scanned with SPM contact mode (NanoWizard, JPK instruments). Ultrafiltration, ultracentrifugation, HIV-1-specific libraries immobilized on supermacroporous epoxy-activated cryogel RP (Protista Biotechnology) and SDS-PAGE, consequently, were used for viral proteins concentration. One-dimensional LC-MS-MS Esquire6000Plus (Bruker Daltonics) tryptic cleavage virus peptide's identification was analyzed with Scaffold (UK) and PEAKS (Canada) software complexes and NCBI database. 64 ScFv phage clones were analyzed in crisscross ELISA tests for HIV specificity. ScFv libraries enriched on diagnostic recombinant HIV peptides gp110 and gp160 provided higher (≥96 percent, p ≤ 0.003) specificity to native HIV proteins from patients isolates than libraries after panning at laboratory U455 proteins. Therefore gp110-gp160 phage-presented ScFv were immobilized at cryogel column and concentrated native HIV-1 subtype A proteins in RP successfully. Recombinant M13 has symmetric ScFv ''head'' structure at SPM images. Gp120 and gp41 peptide's LC-MS-MS identification was possible only in samples run through all concentration stages including reverse panning and was not possible in samples without RP. Best RP column eluate fractions were detected in WB. PEAKS complex can be used for HIV envelop peptides quantitative and variability analysis, Scaffold's potency is modest for it. Proteomics databases search revealed the predominance of HIV-1 subtype A Env, Pol and Gag tryptic peptides in native viral samples. The amount of the particular HIV-1 protein variant in the LC-MS sample correlated with the probability of its detection by this method. RP concentration approach can be used for envelop variation's sequence identification of predominating native HIV-1 quasispecies, circulating in the bloodstream. We suppose RP technique and LC--MS-MS as crucial identification methods for HIV preventive vaccines creation.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".