Evaluation of D<sub>10</sub>-Leu Metabolic Labeling Coupled with MALDI−MS Analysis in Studying the Response of the Yeast Proteome to H<sub>2</sub>O<sub>2</sub> Challenge
Bibliographic record
Abstract
An efficient D 10 -Leu metabolic-labeling method combined with isotope-ratio quantitation by MALDI-TOF MS was used to probe the response of the yeast proteome to H 2 O 2 . Control cultures correct for effects not associated with H 2 O 2 challenge. A stress-response index to H 2 O 2 (SRI H 2 O 2 ) is defined, and values are reported for seven proteins at 45−225 min following exposure to 0.4 mM H 2 O 2 . The time course of protein accumulation in unstressed cells following the H 10 - to D 10 -SCD switch suggests that proteome responses at <45 min could be monitored by addition of excess D 10 -Leu to H 10 -cultures. Keywords: metabolic labeling • D 10 -Leu • 2DE • MALDI-TOF MS • S. cerevisiae • H 2 O 2 • SRI H 2 O 2
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.021 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.002 | 0.006 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.002 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".