Haemolymph levels of <i>Schistocerca gregaria</i> ion transport peptide and ion transport‐like peptide
Bibliographic record
Abstract
Abstract. Titres of ion transport peptide (ITP) and ion transport‐like peptide (ITP‐L) in the haemolymph of the desert locust Schistocerca gregaria Forskål are investigated using a combination of enzyme‐linked immunosorbent assay and reversed‐phase high‐performance liquid chromatography (HPLC). The estimated circulating levels of these two peptides are used to investigate their putative physiological roles. Haemolymph levels of ITP‐L are significantly higher in fed and exercise‐stressed locusts than in starved insects, suggesting a role for ITP‐L in postfeeding osmoregulation and metabolism associated with stress. The higher titres of ITP‐L in the final nymphal stadium could be related to increased levels of ecdysteroids over this same period. In addition detection of ITP‐L in the brain and corpus cardiacum of the locust demonstrates for the first time that the ITP‐L is expressed in these tissues. Immunoreactivity to antibodies raised against ITP is significantly higher in fed locusts compared with starved insects. However, >95% of this immunoreactivity elutes later than synthetic ITP when separated by HPLC. The results suggest that this more hydrophobic immunoreactivity is neither native ITP nor a metabolic breakdown product of ITP. Haemolymph levels of immunoreactivity to ITP during the last nymphal stadium are similar to ITP‐L but, unlike ITP‐L, there is no measured increase in immunoreactivity to ITP due to exercise stress.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".