Molecular Cloning and Phylogenetic Analysis of Two Plant-Parasitic Nematode 14-3-3 Genes
Bibliographic record
Abstract
Full-length cDNA sequences of highly conserved ubiquitous 14-3-3 proteins were cloned from plant parasitic Heterodera glycines and Meloidogyne incognita using RT-PCR and RACE methods. The two genes were named as Hgly2 and Minc3, respectively. Hgly2 consisted of nucleotide sequence of 1027bp and Minc3 of 1525 bp. And the open reading frames (ORF) encode peptide of 251 and 261 amino acids separately. Homology analysis showed that the deduced amino acid sequences shared the high homology with different nematode species. The phylogenetic analysis indicated that the proteins from plant parasitic nematode were more similar to insect proteins than plant and other animal proteins reported by previous research.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".