Effect of Removing the Amino-Terminal Hexapeptide of Tropomyosin on the Properties of the Thin Filament
Bibliographic record
Abstract
The role of the amino-terminal region of alpha-striated muscle tropomyosin has been analyzed by reconstituting thin filaments with a version of the protein lacking the first six amino acids. While Omp T-digested tropomyosin (residues 7-284) does not bind significantly to F-actin at micromolar concentrations, an interaction is induced by skeletal troponin. At a moderate ionic strength (50 mM KCl), the apparent Kd values are 0.26 microM (with EGTA) and 1.6 microM (with Ca2+). At higher neutral salt (140 mM KCl), reconstitution is observed in the micromolar range only at high pCa (Kd = 1.3 microM). However, when chloride is replaced by acetate, the binding isotherms reach saturation under both extremes of Ca2+ [apparent Kd values of 0.32 microM (with EGTA) and 2.6 microM (with Ca2+)]. The induction of binding of truncated tropomyosin to F-actin by troponin is attributable, in part, to troponin-I, but whereas the amino-terminal fragment of troponin-T (N-Tn-T, residues 1-158) enhances the effect of troponin-I in the case of other tropomyosin products specifically, unacetylated tropomyosin (residues 1-284), and carboxypeptidase-digested tropomyosin (residues 1-273) [Heeley, D. H., et al. (1987) J. Biol. Chem. 262, 9971-9978], it is ineffective with regard to Omp T-digested tropomyosin, suggesting that cleavage has disrupted a binding site for this section of troponin-T. Thin filaments (with Ca2+) containing Omp T-digested tropomyosin activate the steady-state myosin-MgATPase to a greater extent than the integral system, consistent with the interaction between N-Tn-T and the amino-terminal region of tropomyosin having a regulatory function. At high pCa, the truncated system exhibits a less cooperative interaction with myosin-S1-ADP but the affinity for the ligand is stronger. In context with the current methodologies, the consequences of shortening tropomyosin at one end as opposed to the other are the reverse of each other.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".