These abstracts have been selected for presentation in 4 sessions throughout the meeting. Please refer to the PROGRAM for more details.
Bibliographic record
Abstract
Background: Altered metabolism is thought to play an important role in the pathogenesis of heart failure.Study of the metabolism may provide insights into the pathology of heart failure and may provide new diagnostic tools.Proton magnetic resonance spectroscopy (MRS) allows us to quantify total creatine, which plays an essential role in the transport of energy from the mitochondria to the myofibrils.Experimental autoimmune myocarditis (EAM) in rodents is an accepted model of myocarditis and dilated cardiomyopathy.As of yet, proton MRS has not been used to study the changes occurring in this model of heart failure.We aimed to study the metabolic changes occurring in an animal model of EAM, and compare these to the findings in healthy animals.Methods: Myocardial tissue of 10 male young Lewis rats with EAM (35 days after immunization with 0.25 mg porcine myocardial myosine) was analysed using 1H-MAS-MRS (Bruker 600 MHz).The metabolic profile was compared to fresh (n ¼ 7) and frozen (n ¼ 8) healthy controls and to the results from histology and immunohistochemistry (CD68).For fresh control samples the spectra were taken less than 10 min after death.Frozen control samples and myocarditis samples were shock-frozen in liquid nitrogen and stored for 4-6 months at -808C before measurements.Myocardial tissue from a basalcavity slice of the the left ventricle (30-40 mg) excluding epicardial tissue was placed in a 4 mm zirconium rotor, packed homogeneously using a spacer and spun at 4 kHz at 293 K.A water suppression pulse sequence was applied to obtain the proton spectrum (ns ¼ 128, t ¼ 7 min).Spectra were phased and baseline correction using polynomial fit to the region of interest was applied before integration of the peaks.Results: The metabolic ratio of taurine to creatine obtained by spectral analysis proved to be a significant biomarker for diagnosis of myocarditis compared to healthy controls (taurine/creatine ratio in myocarditis: 4.47(+0.83),fresh control: 2.59(+0.09),frozen control: 2.59(+0.28);P , 0.001).Myocarditis was confirmed histologically with an inflammatory cellular infiltrate and CD68 positive staining.Conclusions: Myocardial taurine/creatine ratio as detected by proton MRS is able to differentiate between healthy myocardium and myocardium from rats with EAM.This variation may occur due to creatine depletion as described in heart failure and/or an increase in taurine due to its antioxidant activity in inflammatory reactions.1237
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.005 |
| Meta-epidemiology (narrow) | 0.003 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.002 | 0.000 |
| Scholarly communication | 0.005 | 0.002 |
| Open science | 0.003 | 0.005 |
| Research integrity | 0.003 | 0.002 |
| Insufficient payload (model declined to judge) | 0.634 | 0.487 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; the direct Gemma label and the distilled Codex classifier agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".