1621 EXERCISE PREVENTS BLADDER DYSFUNCTION IN THE TYPE 2 DIABETIC MOUSE MODEL

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Urodynamic Testing1 Apr 20101621 EXERCISE PREVENTS BLADDER DYSFUNCTION IN THE TYPE 2 DIABETIC MOUSE MODEL Manasi Vadhavkar, Lynn Stothers, and Ismail Laher Manasi VadhavkarManasi Vadhavkar More articles by this author , Lynn StothersLynn Stothers More articles by this author , and Ismail LaherIsmail Laher More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1401AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Type 2 diabetes accounts for more than 90% of diabetes mellitus. Detrusor dysfunction is a frequent complication of diabetes and can lead to a variety of lower urinary tract symptoms (LUTS). Exercise is the initial recommended management strategy in many diabetics and has been shown to prevent or reduce muscle dysfunction in other areas of the body; but its effect on prevention of detrusor dysfunction is unknown. The objective of this study was to determine if the introduction of moderate exercise early in the onset of type 2 diabetes would prevent bladder dysfunction and reduce voiding frequency in the db/db mouse, a frequently used animal model of type 2 diabetes. METHODS A randomised trial of 24 db/db female mice (BKS. Cg-m+/+Leprdb/ J strain) and their age matched wild type controls (WT) were used. Mice were equally divided into sedentary and exercise groups. Exercise consisted of one hour daily treadmill drills for 8 weeks at a speed of 5.2 meters/min. Ambulatory urodynamic protocols using suprapubic cystostomy were performed with water infusion to observe for overactivity, functional volume and pressure flow in all cohorts. Pad voiding pattern tests, contractile strength and reactivity of isolated detrusor strips in WT and db/db mice, in both sedentary and exercise groups were compared pharmacologically. RESULTS Diabetes increased the frequency of voiding, bladder capacity and residual volume. Exercise decreased voiding frequency in db/db mice where the voiding frequency was 5.8 ± 0.5 (db/db exercise) vs.10.8 ± 1.1 (db/db control) (t-test, P < 0.001). In cystometric analysis, the bladder capacity of db/db sedentary mice was 0.27 ± 0.05 ml compared to 0.14 ± 0.02 ml in the db/db exercise group (t- test, P < 0.05). Micturition pressure was higher in the db/db sedentary mice (30.69 ± 1.51 cm H2O vs. 25.61 ± 1.13 cm H2O after exercise, t- test, P < 0.05). Isolated strips of bladder muscle from sedentary db/db mice were more responsive to carbachol than strips from db/db exercise mice. This moderate level of exercise did not alter the body weight or blood glucose levels of db/db mice. CONCLUSIONS Moderate routine exercise resulted in detrusor function in type 2 diabetic mice statically comparable to non diabetic controls. Reduced voiding frequency and improved urodynamic parameters including reduction in overactivity and improved emptying were observed benefits; complications of which were statistically higher in the sedentary diabetic group. These findings suggest a potentially preventable component to diabetic detrusor dysfunction which could form the foundation for human studies. Vancouver, Canada© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e626 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Manasi Vadhavkar More articles by this author Lynn Stothers More articles by this author Ismail Laher More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...

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