Characterization of a Bacteriocin Produced by Lactobacillus plantarum Lp6SH Isolated from “Sha’a”, a Maize-Based Traditionally Fermented Beverage from Cameroon
Bibliographic record
Abstract
Lactobacillus plantarum Lp6SH isolated from “Sha’a” a maize-based traditionally fermented beverage from Cameroon, produces a bacteriocin active against Gram-positive and Gram-negative bacteria including Listeria innocua, Staphylococcus aureus, Salmonella typhi, Bacillus cereus, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Shigella flexneri. Highest bacteriocin production in buffered MRS broth medium was achieved after 6 h of incubation without agitation. The bacteriocin is of proteinaceous nature as judged by the complete loss of activity after treatment by proteolytic enzymes. Interestingly, this bacteriocin was stable at 121°C for 30 min, over a wide range of pH (2.0-10.0), and when treated with surfactants, organic solvents, EDTA and NaCl. The activity remained after 8 months of storage at 4°C and the mode of action against Salmonella typhi ATCC 6539 was bactericidal. The bacteriocin was partially purified using ammonium sulphate precipitation, gel filtration and cation exchange chromatographies. Mass Spectrometry Analysis showed that the Molecular weight of the bacteriocin is 2.340 kDa.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".