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Record W1975658035 · doi:10.1080/01694243.2012.693830

Study on the role of polyethylenimine as gene delivery carrier using molecular dynamics simulations

2012· article· en· W1975658035 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueJournal of Adhesion Science and Technology · 2012
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRNA Interference and Gene Delivery
Canadian institutionsUniversity of Alberta
FundersWestern Canada Research Grid
KeywordsPolyethylenimineNucleic acidGene deliveryMolecular dynamicsMoleculePolymerNanotechnologyMaterials scienceDNACombinatorial chemistryBiophysicsChemistryGenetic enhancementComputational chemistryOrganic chemistryTransfectionGeneBiochemistryBiology

Abstract

fetched live from OpenAlex

Polyethylenimine (PEI) is known to be one of the most promising polymers to serve as a delivery carrier in gene therapy. Complexation between PEI molecules and nucleic acids results in nanoparticles that can subsequently enter cells for therapeutic treatment. Design of the carrier molecules for stabler complexation, better cell uptake, and lower cytotoxicity remains to be a challenge. Molecular dynamics (MD) simulations allow atomistic level examination of the complexation between PEI and nucleic acids, as well as the interaction of the complexes formed with other entities in the delivery path. It is a powerful tool in revealing the role of carrier molecules and guiding the design of more effective delivery systems. In this work, we first review the current status in studying complexation of PEI with nucleic acids and interaction between the complex formed and cell membrane. We have conducted preliminary MD simulations on several aspects of complexation between PEI and DNA. These results are presented to demonstrate how MD simulations can help understand the role of PEI in serving as a gene delivery carrier.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.018
Threshold uncertainty score0.220

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.290
Teacher spread0.273 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it