Mutations in the Dimerization Domain of the b Subunit from the Escherichia coli ATP synthase

The b subunit dimer of Escherichia coli ATP synthase serves essential roles as an assembly factor for the enzyme and as a stator during rotational catalysis. To investigate the functional importance of its coiled coil dimerization domain, a series of internal deletions including each individual residue between Lys-100 and Ala-105 (bΔK100-bΔA105), bΔK100-A103, and bΔK100-Q106 as well as a control bK100A missense mutation were prepared. All of the mutants supported assembly of ATP synthase, but all single-residue deletions failed to support growth on acetate, indicating a severe defect in oxidative phosphorylation, and bΔK100-Q106 displayed moderately The of a in to of of dimerization of the of and the deletions dimerization for all bΔK100-Q106 the dimerization of b but dimerization of and were in dimerization of b in assembly of ATP synthase in The of single-residue deletions in b the b subunit serves an in on to the to on between the b and The b subunit dimer of Escherichia coli ATP synthase serves essential roles as an assembly factor for the enzyme and as a stator during rotational catalysis. To investigate the functional importance of its coiled coil dimerization domain, a series of internal deletions including each individual residue between Lys-100 and Ala-105 (bΔK100-bΔA105), bΔK100-A103, and bΔK100-Q106 as well as a control bK100A missense mutation were prepared. All of the mutants supported assembly of ATP synthase, but all single-residue deletions failed to support growth on acetate, indicating a severe defect in oxidative phosphorylation, and bΔK100-Q106 displayed moderately The of a in to of of dimerization of the of and the deletions dimerization for all bΔK100-Q106 the dimerization of b but dimerization of and were in dimerization of b in assembly of ATP synthase in The of single-residue deletions in b the b subunit serves an in on to the to on between the b and in and the enzyme synthase for the of The the of the of of as the enzyme Escherichia the in the and the of the subunit The of of the enzyme the and of the to the of ATP The of b subunit of the of ATP synthase to as the and as a the in during of the The b subunit an dimer the the the dimerization and the as the to the of of the the the dimerization domain, b a coiled coil coiled coiled a in a the of the individual a the of a and and of of a and the the of the dimerization the the in the in coiled between the to in the of in a and the of b dimerization to of coiled to an in the dimerization of b and to The of the in ATP synthase functional importance of deletions of a of between and of to and of to were the mutants of of the of the the dimerization severe an and to in the b dimer of ATP synthase and the of the of the b dimerization functional the a series of single-residue between Lys-100 and Ala-105 in the dimerization and residue residue Lys-100 were enzyme and dimerization of b were of assembly of ATP The of the of the b dimer the of dimerization and b a as a stator and were as were to the of for all for b the were the of to the of and as were the were in a and The a and of the of the the of were and for ATP and as were in and for were to the for the of the and a of the b subunit the for all b all of b were of b an to the in and the were and in the b the in of b b were in the growth on as the as All b were as the b were in of of and of were and were and in a to in to and were a The in a for The and a the The were a of in the were and to on a were a and a of in the a and were were were were for and to were were the were the of and and and the were for a a the of b of The for and of of of b were to and an the b subunit of ATP To for dimerization of of to the were to the to the a of b to a b for the of as of and of the were in a of and and for b subunit to to the The were to the the and the were in of of the and investigate the importance of the coiled coil in the dimerization of the b subunit of ATP synthase, a series of b mutants were deletions in the of to in the b subunit To the were to of of a series of single-residue deletions as well as deletions and bΔK100-Q106 and a control bK100A The deletions of and of the and were to severe on the coiled coil the single-residue All mutants were in the b subunit and in the The were the a of the b subunit of to of b a of b on b mutants were for to support oxidative growth on as the All of the single-residue deletions growth on acetate, indicating an to an The support growth on indicating a functional ATP synthase, the bΔK100-Q106 growth to were the of the of of b of of in a of b on ATP the the b mutants on the assembly and of ATP synthase, were and the b and of ATP synthase b and in the the all b of of b and to in the indicating enzyme assembly of b of were were and of b on of ATP for and The single-residue deletions all in the ATP of each individual residue between Lys-100 and Ala-105 to on oxidative and enzyme the to roles of individual but to the coiled coil on the for a bK100A as a for in the of and of the b mutants in the to in assembly were To for in the of ATP synthase in the the of ATP were the of a in the between the b and the indicating the b on the of the in the the mutants in the the as the in growth the the the bΔK100-Q106 and the The single-residue deletions between and and between the b deletions on the assembly of ATP synthase, in of the to the bK100A mutation on the of ATP the the residue of synthase were to the and as the of were to the and as the of in a To for of ATP to of the were the control the b and bK100A control a the to of in the bΔK100-A103, and bΔK100-Q106 all of in the the b of were in of and to and were and the ATP and were to and b bK100A bΔK100-Q106 and in b the of deletions on the dimerization of the were a of the of and To of b to as b an of the of of the a a of bK100A an of a of the a of the of in a the of in the mutants in to a dimer in an of and a of and bΔK100-Q106 of and of and the of to support oxidative in and the of the the bΔK100-Q106 to the of the of b on the of the the of b and of the the during the for a as the for of b of an as on the of the the of b and of the the during the on the of the the of b and of the the during the of b of an as on the of the the of b and of the the during the for a as the for in a To the on the dimerization of and to the in the and of The of a of b of to in dimerization b of indicating dimerization bK100A a dimer the of bΔK100-Q106 were of a to the and the of failed to indicating a severe of The moderately the of were the the of dimerization in and dimerization of b b were in for and the the of to a of b were to to and an the b subunit of ATP All b of investigate b mutants to were as b mutants b to an for the of a control b of the the The of to the of b to and of b to were the for the of to the and bΔK100-Q106 the to a in the of the the on dimerization the in in in The of b to an as The of to control to the b mutants were in the to the for the of and of the in the were in the as a control for of in the and were to the of to the of the b subunit dimerization on its deletions were in the domain, the of the dimerization domain, were to the of the in the dimerization domain, The of residue Lys-100 to in a of ATP synthase to the enzyme but failed to support growth on a a of oxidative the of b in the assembly and of ATP synthase, its the subunit of well The the b dimer to and a functional for the coiled coil of the b dimerization during rotational catalysis. of the and the mutants to the b dimerization and in and ATP synthase assembly and in of the in a coiled coil for b a single-residue to the on the b subunit dimerization and assembly of ATP of of the the of the and on assembly and the and bΔK100-Q106 were of oxidative phosphorylation, but a single-residue between Lys-100 and Ala-105 were the dimerization on growth and enzyme the bΔK100-Q106 to oxidative phosphorylation, a growth on an and to the on a coiled bΔK100-Q106 to in the of and to in b a coiled of b to in but to of ATP dimerization of the b subunit well its to to and b dimerization to for the assembly of the between b dimerization and in as the and the dimerization the to the b in the and the in the of a between b in and the in of the in the of and bΔK100-Q106 mutants to to to the of mutants assembly of an ATP synthase in dimerization to for assembly in the of the the b on the and dimerization an bΔK100-Q106 the of the in to growth on acetate, the single-residue deletions dimerization the of the in of b to the of dimerization of b essential for to the of dimerization of b mutants as for but for bΔK100-A103, and for the for to of the on the of b on of the the in the to were its an for the deletions in the Lys-100 to and to the The the of the of the to the b in a the the single-residue to the for the in of the b in b a in ATP synthase as a stator the to the a subunit of during the of The a and the between and the of the b subunit to on to single-residue deletions in the dimerization of b an ATP synthase support growth oxidative mutants of an to the the ATP synthase and b a in b to but on and between the b and The moderately the of in to in the the single-residue deletions ATP the in the the b subunit a for and the an of of a the the the in the a and a to to support of b dimerization a the of the in the to the an of and an for the of the in and the enzyme synthase for the of The the of the of of as the enzyme Escherichia the in the and the of the subunit The of of the enzyme the and of the to the of ATP The of The b subunit of the of ATP synthase to as the and as a the in during of the The b subunit an dimer the the the dimerization and the as the to the of of the the the dimerization domain, b a coiled coil coiled coiled a in a the of the individual a the of a and and of of a and the the of the dimerization the the in the in coiled between the to in the of in a and the of b dimerization to of coiled to an in the dimerization of b and to The of the in ATP synthase functional importance of deletions of a of between and of to and of to were the mutants of of the of the the dimerization severe an and to in the b dimer of ATP synthase and the of the of the b dimerization functional the a series of single-residue between Lys-100 and Ala-105 in the dimerization and residue residue Lys-100 were enzyme and dimerization of b were of assembly of ATP The of the of the b dimer the of dimerization and b a as a stator and were as were to the of for all for b the were the of to the of and as were the were in a and The a and of the of the the of were and for ATP and as were in and for were to the for the of the and a of the b subunit the for all b all of b were of b an to the in and the were and in the b the in of b b were in the growth on as the as All b were as the b were in of of and of were and were and in a to in to and were a The in a for The and a the The were a of in the were and to on a were a and a of in the a and were were were were for and to were were the were the of and and and the were for a a the of b of The for and of of of b were to and an the b subunit of ATP To for dimerization of of to the were to the to the a of b to a b for the of as of and of the were in a of and and for b subunit to to the The were to the the and the were in of of the were as were to the of for all for b the were the of to the of and as were the were in a and The a and of the of the the of were and for ATP and as were in and for were to the for the of the and a of the b subunit the for all b all of b were of b an to the in and the were and in the b the in of b b were in the growth on as the as All b were as the b were in of of and of were and were and in a to in to and were a The in a for The and a the The were a of in the were and to on a were a and a of in the a and were were were were for and to were were the were the of and and and the were for a a the of b of The for and of of of b were to and an the b subunit of ATP To for dimerization of of to the were to the to the a of b to a b for the of as of and of the were in a of and and for b subunit to to the The were to the the and the were in of of the and investigate the importance of the coiled coil in the dimerization of the b subunit of ATP synthase, a series of b mutants were deletions in the of to in the b subunit To the were to of of a series of single-residue deletions as well as deletions and bΔK100-Q106 and a control bK100A The deletions of and of the and were to severe on the coiled coil the single-residue All mutants were in the b subunit and in the The were the a of the b subunit of to of b a of b on b mutants were for to support oxidative growth on as the All of the single-residue deletions growth on acetate, indicating an to an The support growth on indicating a functional ATP synthase, the bΔK100-Q106 growth to were the of the of of b of of in a of b on ATP the the b mutants on the assembly and of ATP synthase, were and the b and of ATP synthase b and in the the all b of of b and to in the indicating enzyme assembly of b on of ATP for and The single-residue deletions all in the ATP of each individual residue between Lys-100 and Ala-105 to on oxidative and enzyme the to roles of individual but to the coiled coil on the for a bK100A as a for in the of and of the b mutants in the to in assembly were To for in the of ATP synthase in the the of ATP were the of a in the between the b and the indicating the b on the of the in the the mutants in the the as the in growth the the the bΔK100-Q106 and the The single-residue deletions between and and between the b deletions on the assembly of ATP synthase, in of the to the bK100A mutation on the of ATP the the residue of synthase were to the and as the of were to the and as the of in a To for of ATP to of the were the control the b and bK100A control a the to of in the bΔK100-A103, and bΔK100-Q106 all of in the the b of were in of and to and were and the ATP and were to and b bK100A bΔK100-Q106 and in b the of deletions on the dimerization of the were a of the of and To of b to as b an of the of of the a a of bK100A an of a of the a of the of in a the of in the mutants in to a dimer in an of and a of and bΔK100-Q106 of and of and the of to support oxidative in and the of the the bΔK100-Q106 to the of the of b on the of the the of b and of the the during the for a as the for of b of an as on the of the the of b and of the the during the on the of the the of b and of the the during the of b of an as on the of the the of b and of the the during the for a as the for in a To the on the dimerization of and to the in the and of The of a of b of to in dimerization b of indicating dimerization bK100A a dimer the of bΔK100-Q106 were of a to the and the of failed to indicating a severe of The moderately the of were the the of dimerization in and dimerization of b b were in for and the the of to a of b were to to and an the b subunit of ATP All b of investigate b mutants to were as b mutants b to an for the of a control b of the the The of to the of b to and of b to were the for the of to the and bΔK100-Q106 the to a in the of the the on dimerization the in in in The of b to an as The of to control to the b mutants were in the to the for the of and of the in the were in the as a control for of in the and and investigate the importance of the coiled coil in the dimerization of the b subunit of ATP synthase, a series of b mutants were deletions in the of to in the b subunit To the were to of of a series of single-residue deletions as well as deletions and bΔK100-Q106 and a control bK100A The deletions of and of the and were to severe on the coiled coil the single-residue All mutants were in the b subunit and in the The were the a of the b subunit of to of b a of b on b mutants were for to support oxidative growth on as the All of the single-residue deletions growth on acetate, indicating an to an The support growth on indicating a functional ATP synthase, the bΔK100-Q106 growth to were the of the of of b on ATP the the b mutants on the assembly and of ATP synthase, were and the b and of ATP synthase b and in the the all b of of b and to in the indicating enzyme assembly of b on of ATP for and The single-residue deletions all in the ATP of each individual residue between Lys-100 and Ala-105 to on oxidative and enzyme the to roles of individual but to the coiled coil on the for a bK100A as a for in the of and of the b mutants in the to in assembly were To for in the of ATP synthase in the the of ATP were the of a in the between the b and the indicating the b on the of the in the the mutants in the the as the in growth the the the bΔK100-Q106 and the The single-residue deletions between and and between the b deletions on the assembly of ATP synthase, in of the to the bK100A mutation on the of ATP the the residue To for of ATP to of the were the control the b and bK100A control a the to of in the bΔK100-A103, and bΔK100-Q106 all of in the in b the of deletions on the dimerization of the were a of the of and To of b to as b an of the of of the a a of bK100A an of a of the a of the of in a the of in the mutants in to a dimer in an of and a of and bΔK100-Q106 of and of and the of to support oxidative in and the of the the bΔK100-Q106 to the of the To the on the dimerization of and to the in the and of The of a of b of to in dimerization b of indicating dimerization bK100A a dimer the of bΔK100-Q106 were of a to the and the of failed to indicating a severe of The moderately the of were the the of dimerization in and dimerization of b investigate b mutants to were as b mutants b to an for the of a control b of the the The of to the of b to and of b to were the for the of to the and bΔK100-Q106 the to a in the of the the on dimerization the in in in were to the of to the of the b subunit dimerization on its deletions were in the domain, the of the dimerization domain, were to the of the in the dimerization domain, The of residue Lys-100 to in a of ATP synthase to the enzyme but failed to support growth on a a of oxidative the of b in the assembly and of ATP synthase, its the subunit of well The the b dimer to and a functional for the coiled coil of the b dimerization during rotational catalysis. of the and the mutants to the b dimerization and in and ATP synthase assembly and in of the in a coiled coil for b a single-residue to the on the b subunit dimerization and assembly of ATP of of the the of the and on assembly and the and bΔK100-Q106 were of oxidative phosphorylation, but a single-residue between Lys-100 and Ala-105 were the dimerization on growth and enzyme the bΔK100-Q106 to oxidative phosphorylation, a growth on an and to the on a coiled bΔK100-Q106 to in the of and to in b a coiled of b to in but to of ATP dimerization of the b subunit well its to to and b dimerization to for the assembly of the between b dimerization and in as the and the dimerization the to the b in the and the in the of a between b in and the in of the in the of and bΔK100-Q106 mutants to to to the of mutants assembly of an ATP synthase in dimerization to for assembly in the of the the b on the and dimerization an bΔK100-Q106 the of the in to growth on acetate, the single-residue deletions dimerization the of the in of b to the of dimerization of b essential for to the of dimerization of b mutants as for but for bΔK100-A103, and for the for to of the on the of b on of the the in the to were its an for the deletions in the Lys-100 to and to the The the of the of the to the b in a the the single-residue to the for the in of the b in b a in ATP synthase as a stator the to the a subunit of during the of The a and the between and the of the b subunit to on to single-residue deletions in the dimerization of b an ATP synthase support growth oxidative mutants of an to the the ATP synthase and b a in b to but on and between the b and The moderately the of in to in the the single-residue deletions ATP the in the the b subunit a for and the an of of a the the the in the a and a to to support of b dimerization a the of the in the to the an of and an for the of the The were to the of to the of the b subunit dimerization on its deletions were in the domain, the of the dimerization domain, were to the of the in the dimerization domain, The of residue Lys-100 to in a of ATP synthase to the enzyme but failed to support growth on a a of oxidative the of b in the assembly and of ATP synthase, its the subunit of well The the b dimer to and a functional for the coiled coil of the b dimerization during rotational catalysis. of the and the mutants to the b dimerization and in and ATP synthase assembly and in The of the in a coiled coil for b a single-residue to the on the b subunit dimerization and assembly of ATP of of the the of the and on assembly and the and bΔK100-Q106 were of oxidative phosphorylation, but a single-residue between Lys-100 and Ala-105 were the dimerization on growth and enzyme the bΔK100-Q106 to oxidative phosphorylation, a growth on an and to the on a coiled bΔK100-Q106 to in the of and to in b a coiled of b to in but to of ATP dimerization of the b subunit well its to to and b dimerization to for the assembly of the between b dimerization and in as the and the dimerization the to the b in the and the in the of a between b in and the in of the in the of and bΔK100-Q106 mutants to to to the of mutants assembly of an ATP synthase in dimerization to for assembly in the of the the b on the and dimerization an bΔK100-Q106 the of the in to growth on acetate, the single-residue deletions dimerization the of the in of b to the of dimerization of b essential for to the of dimerization of b mutants as for but for bΔK100-A103, and for the for to of the on the of b on of the the in the to were its an for the deletions in the Lys-100 to and to the The the of the of the to the b in a the the single-residue to the for the in of the b in b a in ATP synthase as a stator the to the a subunit of during the of The a and the between and the of the b subunit to on to single-residue deletions in the dimerization of b an ATP synthase support growth oxidative mutants of an to the the ATP synthase and b a in b to but on and between the b and The moderately the of in to in the the single-residue deletions ATP the in the the b subunit a for and the an of of a the the the in the a and a to to support of b dimerization a the of the in the to the an of and an for the of the to and for the of and and of the of for the of the of the were the of and supported a and the of

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.