Cloning of Bovine Eukaryotic Translation Initiation Factor 4E (eIF-4E) and Its Expression in the Bovine Mammary Gland at Different Physiological Stages
Bibliographic record
Abstract
Bovine eIF-4E cDNA was cloned and its expression in the bovine mammary gland at distinct physiological stages was investigated. Bovine eIF-4E cDNA and protein sequences are highly homologous to those from other mammals. Using Northern blot analysis, we did not detect eIF-4E expression in the prepubertal bovine mammary gland, whereas a low level of eIF-4E mRNA was observed in the mammary tissues of heifers during the third estrous cycle. The eIF-4E mRNA level was significantly higher in the lactating mammary gland compared to the mammary tissues obtained from heifers during the third estrous cycle. Alteration of eIF-4E expression in bovine mammary tissues during different physiological stages indicates the involvement of eIF-4E in mammary development. Elevated eIF-4E expression during lactation may be related to increased translation of certain mRNA or the acceleration of overall protein synthesis.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".