Sequence and expression of a constitutive, facilitated glucose transporter (GLUT1) in Atlantic cod<i>Gadus morhua</i>
Bibliographic record
Abstract
A putative glucose transporter, GLUT1, is reported for Atlantic cod Gadus morhua. A combination of RT-PCR, RLM-RACE and genome walking were used to articulate a 4560 bp cDNA (GenBank accession number AY526497). It contains a 149 bp 5' UTR, a 1470 bp open reading frame and a 2941 bp 3' UTR. At the nucleotide level, the cod GLUT1 ORF shares 78.2% sequence identity to human GLUT1 and the deduced amino acid sequence clusters with GLUT1s from rainbow trout and carp. GLUT1 transcript is highly expressed in brain, gill, heart and kidney and expressed to a lower level in at least six other tissues. Expression is evident immediately upon fertilization of eggs. Six hours of hypoxia at 40% DO(2) did not alter expression levels in brain, gill, heart or kidney. The level of expression is not substantially altered in heart during low temperature challenge, although there is a suggestion that colder temperature could lead to lower levels of expression, consistent with the concept that the cold-acclimated heart has a reduced dependence upon glucose as a metabolic fuel. Two months of starvation did not significantly alter the level of expression of GLUT1 in heart. This is in marked contrast to the rat heart where fasting leads to a substantial decrease in GLUT1 levels. Overall, there is a ubiquitous tissue distribution of GLUT1, consistent with other species, and the level of gene expression, especially in heart, is relatively constant over a range of physiological conditions.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".