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Record W1980050211 · doi:10.1042/bst0340858

Regulation of Ras in lymphocytes: get a GRP

2006· article· en· W1980050211 on OpenAlexaff
James C. Stone

Bibliographic record

VenueBiochemical Society Transactions · 2006
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicProtein Kinase Regulation and GTPase Signaling
Canadian institutionsUniversity of Alberta
Fundersnot available
KeywordsGuanine nucleotide exchange factorGTPaseCell biologyDiacylglycerol kinaseBiologySignal transductionProtein kinase C

Abstract

fetched live from OpenAlex

RasGRPs (guanine nucleotide releasing proteins) are a family of four GEFs (guanine nucleotide-exchange factors) (Ras GEFs) that positively regulate Ras and related small GTPases. RasGRP1 possesses a catalytic region consisting of a REM (Ras exchange motif) and a CDC25 (cell division cycle 25) domain. RasGRP1 also possesses a DAG (diacylglycerol)-binding C1 domain and a pair of EF hands that bind calcium. RasGRP1 is selectively expressed in lymphocytes as well as in some cells of the brain, kidney and skin. Functional analysis supports the hypothesis that RasGRP1 serves to couple TCR (T-cell receptor) stimulation and phospholipase C activation with Ras signalling. In B-cells, both RasGRP1 and RasGRP3 play a similar role downstream of the B-cell receptor. RasGRP2 acts on the Ras-related protein Rap and functions in platelet adhesion. RasGRP4 is expressed in mast cells and certain myeloid leukaemia cells. Membrane DAG regulates RasGRPs directly by recruitment to cellular membranes, as well as indirectly by protein kinase C-mediated phosphorylation. The properties of RasGRPs provide a novel view of Ras regulation in lymphocytes and explain several earlier observations. Many experimental results obtained with DAG analogues could be reviewed in light of these findings.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.017

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0050.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.220
Teacher spread0.214 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations79
Published2006
Admission routes1
Has abstractyes

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