Developing a scale-up system for the in vitro multiplication of thidiazuron-induced strawberry shoots using a bioreactor
Bibliographic record
Abstract
The use of large-scale liquid cultures in a bioreactor system has the potential to resolve the manual handling of the various stages of micropropagation and increases shoot multiplication in vitro significantly compared with those cultured on semi-solid gelled medium. In an attempt to improve the micropropagation protocol for strawberry (Fragaria × ananassa Duch.), a procedure for the mass propagation of adventitious shoots regenerated from leaf, sepal and petiole explants of cultivar Bounty using a liquid medium-containing bioreactor system combined with gelled medium is described. Leaf disks, sepals and petiole halves produced multiple buds and shoots without an intermediary callus phase on 2-4 µM thidiazuron (TDZ)-containing shoot induction medium within 5-6 wk of culture initiation. TDZ supported rapid shoot proliferation at low concentrations (0.1 µM), but induced hyperhydricity in a bioreactor system. Bioreactor-multiplied hyperhydric shoots were transferred to gelled medium containing 2-4 µM zeatin, and produced normal shoots and root within 4 wk of culture. In vitro derived plantlets were acclimatized and eventually established in the greenhouse and in the field. Present results suggested the possibility of large-scale multiplication of strawberry shoots in bioreactors. Key words: Fragaria × ananassa, growth regulator, shoot regeneration, RITA ® bioreactor
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".