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Record W1980895620 · doi:10.1002/cmdc.200890000

Cover Picture: CMP‐Pseudaminic Acid is a Natural Potent Inhibitor of PseB, the First Enzyme of the Pseudaminic Acid Pathway in <i>Campylobacter jejuni</i> and <i>Helicobacter pylori</i> (ChemMedChem 1/2008)

2008· paratext· en· W1980895620 on OpenAlexaff
David J. McNally, Ian C. Schoenhofen, R. Scott Houliston, Nam Huan Khieu, Dennis M. Whitfield, Susan M. Logan, Harold C. Jarrell, Jean‐Robert Brisson

Bibliographic record

VenueChemMedChem · 2008
Typeparatext
Languageen
FieldNursing
TopicMicrobial Metabolites in Food Biotechnology
Canadian institutionsInstitute for Biological Sciences
Fundersnot available
KeywordsCampylobacter jejuniHelicobacter pyloriEnzymeChemistryGlycanStereochemistryDocking (animal)MicrobiologyNuclear magnetic resonance spectroscopyBiochemistrySialic acidVirulenceCampylobacterActive siteBiofilmBacteriaBiologyGlycoproteinGeneMedicine

Abstract

fetched live from OpenAlex

The cover picture shows the six enzymes (Pse B, C, H, G, I, F) responsible for producing CMP-pseudaminic acid (CMP-Pse) starting from UDP-GlcNAc in Campylobacter jejuni and Helicobacter pylori. These pathogens modify their flagella with sialic acid-like sugars such as pseudaminic acid (Pse) which are required for flagellar assembly, motility, and hence virulence. Pse B plays a central role in Pse biosynthesis and is also thought to be implicated in other glycan pathways making it a prime therapeutic target. Saturation transfer difference nuclear magnetic resonance spectroscopy (STD NMR) was used to determine binding epitopes for Pse B and to characterize Pse B inhibition with CMP-Pse at the molecular level. Docking studies and CORCEMA calculations validated STD NMR results and revealed that CMP-Pse and UDP-GlcNAc adopt similar conformations within the Pse B active site. These findings will guide the development of small-molecule inhibitors as a means to pharmaceutically control C. jejuni and H. pylori infections. For details, see the Communication by D. J. McNally, et al. on p. 55 ff.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: Not applicable
GenreCandidate signal: Other · Consensus signal: Other
Teacher disagreement score0.250
Threshold uncertainty score0.836

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.001
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0010.001
Open science0.0010.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.2500.070

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.222
Teacher spread0.211 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreOther

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2008
Admission routes1
Has abstractyes

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