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Record W1980895620 · doi:10.1002/cmdc.200890000

Cover Picture: CMP‐Pseudaminic Acid is a Natural Potent Inhibitor of PseB, the First Enzyme of the Pseudaminic Acid Pathway in <i>Campylobacter jejuni</i> and <i>Helicobacter pylori</i> (ChemMedChem 1/2008)

2008· paratext· en· W1980895620 on OpenAlexaff
David J. McNally, Ian C. Schoenhofen, R. Scott Houliston, Nam Huan Khieu, Dennis M. Whitfield, Susan M. Logan, Harold C. Jarrell, Jean‐Robert Brisson

Bibliographic record

VenueChemMedChem · 2008
Typeparatext
Languageen
FieldNursing
TopicMicrobial Metabolites in Food Biotechnology
Canadian institutionsInstitute for Biological Sciences
Fundersnot available
KeywordsCampylobacter jejuniHelicobacter pyloriEnzymeChemistryGlycanStereochemistryDocking (animal)MicrobiologyNuclear magnetic resonance spectroscopyBiochemistrySialic acidVirulenceCampylobacterActive siteBiofilmBacteriaBiologyGlycoproteinGeneMedicine

Abstract

fetched live from OpenAlex

The cover picture shows the six enzymes (Pse B, C, H, G, I, F) responsible for producing CMP-pseudaminic acid (CMP-Pse) starting from UDP-GlcNAc in Campylobacter jejuni and Helicobacter pylori. These pathogens modify their flagella with sialic acid-like sugars such as pseudaminic acid (Pse) which are required for flagellar assembly, motility, and hence virulence. Pse B plays a central role in Pse biosynthesis and is also thought to be implicated in other glycan pathways making it a prime therapeutic target. Saturation transfer difference nuclear magnetic resonance spectroscopy (STD NMR) was used to determine binding epitopes for Pse B and to characterize Pse B inhibition with CMP-Pse at the molecular level. Docking studies and CORCEMA calculations validated STD NMR results and revealed that CMP-Pse and UDP-GlcNAc adopt similar conformations within the Pse B active site. These findings will guide the development of small-molecule inhibitors as a means to pharmaceutically control C. jejuni and H. pylori infections. For details, see the Communication by D. J. McNally, et al. on p. 55 ff.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Science and technology studies, Research integrity
Consensus categoriesMeta-epidemiology (narrow), Research integrity
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.081
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0020.001
Meta-epidemiology (broad)0.0030.001
Bibliometrics0.0000.002
Science and technology studies0.0000.003
Scholarly communication0.0000.000
Open science0.0030.002
Research integrity0.0030.005
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.222
Teacher spread0.211 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; both teacher heads agree on what is shown here.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2008
Admission routes1
Has abstractyes

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