Role of Cysteine Residues in Thiol Modification of Acyl-CoA:Diacylglycerol Acyltransferase 2 from Yeast
Bibliographic record
Abstract
Acyl-CoA:diacylglycerol acyltransferase (EC 2.3.1.20, DGAT or DAGAT), which catalyzes the final step in triacylglycerol biosynthesis, has at least two discrete family members (DGAT1 and DGAT2) with different physiological roles. Here we report a systematic study of the local functional and structural role of seven cysteine residues present in DGAT2 from Saccharomyces cerevisiae (ScDGAT2, also known as Dga1p) using chemical modification in combination with site-directed mutagenesis. We demonstrate that although DGAT2 was susceptible to various thiol-modifying reagents, none of the cysteines were directly involved in the catalytic activity. Analysis of the accessibility of the sulfhydryl groups revealed that cysteines are also not involved in formation of intramolecular disulfide linkages. Inhibition of DGAT activity with thiol-specific reagents was localized to cysteine 314, which was found to be in the proximity of a highly conserved motif of DGAT2. Our work indicates that although this cysteine does not play a role in enzymatic catalysis, it may reside in a crucial position that is near a possible active site of DGAT2 or related to proper folding of the protein.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".