Cryopreservation of Semen from Lake Sturgeon
Bibliographic record
Abstract
Abstract Lake sturgeon Acipenser fulvescens, once abundant and the only species of sturgeon endemic to the North American Great Lakes, is now rare and threatened with extinction as a result of overfishing and habitat destruction. Sperm cryopreservation can be useful for preserving the genetic variability of local populations of lake sturgeon. In this study, we show the conditions of extended storage of unfertilized gametes and successful cryopreservation of lake sturgeon semen using methanol as a cryoprotectant. We demonstrate that unfertilized eggs can be transported for 8–12 h, provided that their initial quality is good. Our results indicate that cryopreservation of lake sturgeon milt caused increased damage to sperm DNA (measured by comet assay); however, some spermatozoa maintained their fertilizing ability. Fertilization with cryopreserved semen caused an 87% reduction in postthaw fertilization rates of prehatched larvae compared with control fertilizations with fresh semen.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".