Studies on Explant Regeneration and Protoplast Culture from Hypocotyl Segments of Jute, Corchorus capsularis L.
Bibliographic record
Abstract
A repeatable protocol was developed for shoot regeneration from hypocotyl explants in jute (C. capsularis) that resulted in 25% regeneration using a combination of 6-BAP (7.5 mgl-1) and adenine hemisulfate (50 mgl-1). Rapid shoot proliferation was observed on medium containing NAA (0.1 mgl-1) and 6-BAP (1 mgl-1). The elongated shoots rooted on basal MS and could be successfully acclimatized, which later developed fertile flowers and viable seeds. After establishing regeneration protocol for jute from hypocotyl explants, the possibility of its response for protoplast isolation and culture was studied. The efficiency of isolation of protoplasts from hypocotyls was influenced by conditions of seedling growth, incubation time in enzyme solution, constitution of the enzyme mixture, osmoticum and purification methods. Isolated non-vacuolated protoplasts when cultured in modified Kao’s medium with 7.2 gl-l sucrose produced microcalli in about four weeks. However, these microcalli did not develop further.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".