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Record W1985168670 · doi:10.1021/jp002761u

A Single-Crystal Electron Paramagnetic Resonance Study at 95 GHz of the Type 1 Copper Site of the Green Nitrite Reductase of <i>Alcaligenes f</i><i>aecalis</i>

2001· article· en· W1985168670 on OpenAlexafffund
Maurice van Gastel, Martin J. Boulanger, Gerard W. Canters, Martina Huber, M.E.P. Murphy, Martin Ph. Verbeet, E. J. J. Groenen

Bibliographic record

VenueThe Journal of Physical Chemistry B · 2001
Typearticle
Languageen
FieldPharmacology, Toxicology and Pharmaceutics
TopicChemical Reactions and Isotopes
Canadian institutionsUniversity of British Columbia
FundersNatural Sciences and Engineering Research Council of CanadaMedical Research Council CanadaNederlandse Organisatie voor Wetenschappelijk Onderzoek
KeywordsNitrite reductaseElectron paramagnetic resonanceCopperNitriteChemistrySingle crystalNuclear magnetic resonanceCrystallographyPhysicsOrganic chemistry

Abstract

fetched live from OpenAlex

An electron-spin-echo−detected electron paramagnetic resonance (EPR) study has been performed for the type 1 copper site of the green nitrite reductase of Alcaligenes faecalis . Accurate g values and principal directions of the g tensor have been obtained. The z principal axis of the g tensor makes an angle of 60° with the direction from copper to the Sδ of the axial methionine. The direction of this axis is compared with that of the blue type 1 proteins azurin and M121Q azurin and of the green type 1 protein M121H azurin, and interpreted in terms of the d orbital at copper in the wave function of the unpaired electron. This d orbital is oriented such that the overlap with the lone-pair orbitals of the ligands is maximum. The relation between the absorption spectrum in the visible, the copper−thiolate interaction, and the rhombicity of the EPR spectrum of type 1 proteins is discussed in terms of the trigonal and tetragonal character of the copper sites and the position and strength of the axial ligand.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.048
GPT teacher head0.355
Teacher spread0.307 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations15
Published2001
Admission routes2
Has abstractyes

Explore more

Same venueThe Journal of Physical Chemistry BSame topicChemical Reactions and IsotopesFrench-language works237,207