Hematological, hemorheological, immunological, and morphological studies of spleen autotransplantation in mice: Preliminary results
Bibliographic record
Abstract
Abstract Using a spleen autotransplantation model, we conducted hematological, hemorheological, immunological, and morphological studies in mice 6 weeks after splenectomy. Sixty male and female A/J inbred mice were equally divided into 3 groups: 1) SE group, splenectomy was performed; 2) AU group, spleen chips were autotransplanted into the omentum without vascular anastomosis following splenectomy; and 3) C group (controls), no intervention in these mice. At postoperative week 6, the following studies were performed: 1) measurement of hematological parameters; 2) hemorheological studies, including relative cell transit time (RCTT) and fibrinogen levels; and 3) activity of peripheral phagocytes, measured by zymozan‐induced chemiluminescence, which was calculated in stimulation index values (SI). In addition, histological investigations of autotransplants were conducted. Erythrocyte mean cell volume and platelet counts, RCTT, fibrinogen levels, and activity of phagocytes were significantly higher in the SE group, compared to those in the C group. In the AU group, these parameters were similar to those in the C group. Morphologically, the transplanted spleen showed normal histology. These data indicate that the transplanted spleens restored their function. We conclude that spleen autotransplantation reserves the normal morphology of spleen and restores most of the spleen's hematological, hemorheological, and immunological functions. Both SI index and erythrocyte deformability can be an informative detection of decreasing splenic function. These data suggest that spleen autotransplantation may provide a useful tool to prevent complications following splenectomy in a clinical setting. Lép autotransplantatios egér modellen a 6. posztoperatív héten haematologiai, haemorheologiai, immunologiai és morphologiai vizsgálatokat végeztünk splenectomiát követően. Hatvan A/J inbred egeret 3 egyenlő csoportba osztottunk: 1) SE csoport: splenectomia, 2) AU csoport: splenectomiát követően lép‐chip autotransplantatio a cseplesz kettőzetébe éranastomosis nélkül, 3) C csoport—control: sebészi beavatkozás nem történt. A 6. posztoperatív héten 1) haematologiai paraméterek meghatározása, 2) a haemorheologiai vizsgálatként a vörösvérsejt deformabilitás mérése (relatív sejttranzit‐idő, RCTT), a fibrinogén szint meghatározása történt, 3) immunologiai mérömódszerként a perifériás phagocyták aktivitását zymosan függő chemiluminescentiával mértük, a stimulációs index (SI) számításával. Párhuzamosan az autotransplantált lép‐chipek szövettani értékelését is elvégeztük. Az erythrocyták átlagos térfogata és a thrombocyták száma, az RCTT és a fibrinogén szint, valamint a phagocyták aktivitása szignifikánsan nagyobb volt az SE, mint a C csoportban. Ezek a paraméterek az AU csoportban hasonlóak voltak a C csoport értékeihez. Az autotransplantált lépek normális histologiájú képet mutattak. Eredményeink azt sugallják, hogy a transplantalt lép‐chipek funkciója helyreállt. Kísérleteink alapján úgy tőnik, hogy a lép autotransplantatio megőrzi a lépszövet normál morphologiáját, nagyrészt helyreállítja a haematologiai, haemorheologiai és immunologiai funkciókat. A lépfunkció csökkenésének érzékeny jelzője lehet az SI és a vörösvérsejt deformabilitás értékeinek változása. Adataink szerint a lépautotransplantatio hatékony eszköz lehet a splenctomiákat követő komplikációk megelőzésében a klinikai gyakorlatban is. © 2003 Wiley‐Liss, Inc. MICROSURGERY 23:483–488 2003
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".