Cloning and Expression Analysis of an Ascorbate Oxidase (AO) Gene from Strawberry (Fragaria×ananassa cv. Toyonaka)
Bibliographic record
Abstract
In this study, a total 1737 bp ascorbate oxidase (AO) gene encoding a 578 amino acid protein was cloned from strawberry (Fragaria×ananassa cv. Toyonaka). Real-time quantitative expression analysis was conducted on FaAO gene of strawberry fruits during 7 developmental stages and in 5 strawberry tissues. The results revealed that during 7 fruit developmental stages, FaAO expressing level gradually increased in yonger stages, and declined dramatically in maturing stages, in which it could barely be detected. According to experimental results, we can conclude that FaAO expresses at a higher level in younger fruits, and lower in latter stages. This could be related to the fact that younger fruits are growing actively, and FaAO has a significant effect on facilitating fruit growth. Results for expression in 5 tissues indicated the highest expression was detected in flowers, and the lowest in fruit tissue (at full red stage). One of the possible reasons is that intense respiration and high ethylene synthesis occur in the mature fruits, repressing the expression of FaAO gene in strawberry fruits. Also, it could be a reason why the ascorbic acid (AA) content in mature fruits is higher than that in other tissues and stages. The differences of expression results among 7 developmental stages and 5 tissues indicated a novel expression pattern of FaAO. We propose that FaAO is correlated with strawberry growth and development, the depression of the FaAO expression can lead to apparent accumulation of AA in strawberry plant.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".