An Expanded Palette of Genetically Encoded Ca <sup>2+</sup> Indicators
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Abstract
Engineered fluorescent protein (FP) chimeras that modulate their fluorescence in response to changes in calcium ion (Ca(2+)) concentration are powerful tools for visualizing intracellular signaling activity. However, despite a decade of availability, the palette of single FP-based Ca(2+) indicators has remained limited to a single green hue. We have expanded this palette by developing blue, improved green, and red intensiometric indicators, as well as an emission ratiometric indicator with an 11,000% ratio change. This series enables improved single-color Ca(2+) imaging in neurons and transgenic Caenorhabditis elegans. In HeLa cells, Ca(2+) was imaged in three subcellular compartments, and, in conjunction with a cyan FP-yellow FP-based indicator, Ca(2+) and adenosine 5'-triphosphate were simultaneously imaged. This palette of indicators paints the way to a colorful new era of Ca(2+) imaging.
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The record
- Venue
- Science
- Topic
- Advanced Fluorescence Microscopy Techniques
- Field
- Biochemistry, Genetics and Molecular Biology
- Canadian institutions
- University of Alberta
- Funders
- Canadian Institutes of Health Research
- Keywords
- Palette (painting)Computational biologyBiologyGeneticsComputer science
- Has abstract in OpenAlex
- yes