Differentiation and molecular detection of Canadian necrotic strains of<i>Potato virus Y</i>
Bibliographic record
Abstract
Isolates of Potato virus Y (PVY) from potatoes grown in Canada were identified as tuber necrotic strains on the basis of biological, serological, and molecular characteristics. Only one isolate, however, caused symptoms of potato tuber necrotic ringspot disease (PTNRD) in progeny tubers of inoculated potato plants Solanum tuberosum 'Nadine' under greenhouse conditions. None of the isolates caused symptoms of PTNRD in potato plants 'Russet Burbank' or 'Saco'. A primer set based on the NIb gene for detection of PVYO by RT-PCR was modified to include detection of the Canadian necrotic isolates of PVY. Restriction analysis of amplicons generated from suspected PVYNTN isolates with primers based on the P1 protein gene differentiated two sets of isolates. Subsequent comparison of the P1 gene sequence of the Canadian isolates with published sequences confirmed that the isolates corresponded to North American and European types of PVYNTN. A new primer pair, based on the NIb gene sequence amplified by RT-PCR, allowed the amplification by RT-PCR of a gene fragment from all PVYN and PVYNTN isolates but not from PVYO strains. These primers proved to be useful for detecting the virus in composite samples of potato leaves, sprouts, and tubers.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".