Isolation and characterization of an aluminum-sensitive mutant of Pseudomonas fluorescens
Bibliographic record
Abstract
An aluminum-sensitive mutant was isolated by chemical mutagenesis using 1-methyl-3-nitro-1-nitrosoguanidine. Although this mutant of Pseudomonas fluorescens was susceptible to aluminum, it did respond in a manner analogous to the wild-type when challenged with either Ca2+, or Ga3+ or Zn2+. However, the aluminum-sensitive mutant did not grow in media with iron. Calcium detoxification appeared to be attained by the elaboration of calcite, a detoxification strategy observed in the wild-type Pseudomonas fluorescens. There was a 40% diminution in biomass when the mutant was cultured in a control medium as compared to the wild-type. The aluminum-sensitive mutant had a characteristic yellow coloration. Electrophoretic analyses of the soluble cellular extract revealed numerous variations between the aluminum-sensitive mutant and wild-type Pseudomonas fluorescens. Bands corresponding to approximate molecular masses of 158 kDa, 116 kDa, 97 kDa, 55 kDa, and 19 kDa were absent in the aluminum-sensitive mutant. Furthermore, bands attributable to molecular masses of 90 kDa and 15 kDa were only evident in the aluminum-sensitive Pseudomonas. It appears that the chemical mutagenesis may have affected only aluminum and iron(III) metabolism in this organism.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".