Detection of the mycoparasite<i>Stachybotrys elegans</i>, using primers with sequence- characterized amplification regions in conventional and real-time PCR
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
A specific and sensitive polymerase chain reaction (PCR) assay was developed for the detection of Stachybotrys elegans, a potential biological control agent and mycoparasite of Rhizoctonia solani. A pair of primers (SE-13F and SE-13R) with sequence-characterized amplification regions was designed and assayed in conventional and real-time PCR to detect S. elegans, in pure cultures and in field soil samples. Following conventional PCR amplification, a product of 880 base pairs was amplified from DNA of all isolates belonging to S. elegans. No product was amplified from DNA of isolates belonging to other species of Stachybotrys, common soil fungi, or bacteria, or from DNA of plant tissue, confirming the specificity of the primers for S. elegans. Stachybotrys elegans was also detected and quantified in field soils, 2 days after its inoculation, using real-time PCR conjugated with the fluorescent SYBR® Green I dye. The assay is reliable and has detected as little as 150 ng of DNA per gram of natural soil. The results of the assay were compared with those of the number of colony-forming units of S. elegans per gram of soil recovered from the same soils. The potential of these specific primers in quantitative PCR assays will facilitate the study of the distribution of the fungus in the soil in field studies of biological control of R. solani.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it