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Record W1992693390 · doi:10.1038/ncomms7442

A RIPK2 inhibitor delays NOD signalling events yet prevents inflammatory cytokine production

2015· article· en· W1992693390 on OpenAlexfundno aff
Ueli Nachbur, Che A. Stafford, Aleksandra Bankovacki, Yifan Zhan, Lisa Lindqvist, Berthe Katrine Fiil, Yelena Khakham, Hyun‐Ja Ko, Jarrod J. Sandow, Hendrik Falk, Jessica K. Holien, Diep Chau, Joanne M. Hildebrand, James E. Vince, Phillip P. Sharp, Andrew I. Webb, Katherine Jackman, Sabrina Mühlen, Catherine L. Kennedy, Kym N. Lowes, James M. Murphy, Mads Gyrd‐Hansen, Michael W. Parker, Elizabeth L. Hartland, Andrew M. Lew, David C.S. Huang, Guillaume Lessène, John Silke

Bibliographic record

VenueNature Communications · 2015
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicInflammasome and immune disorders
Canadian institutionsnot available
FundersNational Health and Medical Research CouncilCanadian Institutes of Health ResearchNovo Nordisk FondenDanmarks Frie ForskningsfondNovo NordiskH. Lundbeck A/SNational Science FoundationLundbeckfondenAustralian Cancer Research FoundationAustralian GovernmentSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
KeywordsNodCytokineSignallingCell biologyProduction (economics)InflammationSignalling pathwaysMedicineBiologyImmunologySignal transductionEndocrinologyDiabetes mellitus

Abstract

fetched live from OpenAlex

Intracellular nucleotide binding and oligomerization domain (NOD) receptors recognize antigens including bacterial peptidoglycans and initiate immune responses by triggering the production of pro-inflammatory cytokines through activating NF-κB and MAP kinases. Receptor interacting protein kinase 2 (RIPK2) is critical for NOD-mediated NF-κB activation and cytokine production. Here we develop and characterize a selective RIPK2 kinase inhibitor, WEHI-345, which delays RIPK2 ubiquitylation and NF-κB activation downstream of NOD engagement. Despite only delaying NF-κB activation on NOD stimulation, WEHI-345 prevents cytokine production in vitro and in vivo and ameliorates experimental autoimmune encephalomyelitis in mice. Our study highlights the importance of the kinase activity of RIPK2 for proper immune responses and demonstrates the therapeutic potential of inhibiting RIPK2 in NOD-driven inflammatory diseases. Receptor Interacting Protein Kinase 2 (RIPK2) mediates innate immune signalling in response to intracellular pathogens, but its aberrant activation contributes to autoimmune pathologies. Here Nachbur et al.describe a RIPK2 inhibitor that is effective in a mouse model of multiple sclerosis.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.578
Threshold uncertainty score0.636

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.275
Teacher spread0.259 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations152
Published2015
Admission routes1
Has abstractyes

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