Saline Contamination of University of Wisconsin Solution Preserved Human Pancreata
Bibliographic record
Abstract
The use of University of Wisconsin (UW) solution is widely accepted for the cold storage and transport of human donor pancreata destined for islet isolation. While several variables exist in islet isolation, proper procurement and packaging is essential for optimizing preprocessing conditions. This study examined the effect of normal saline (NS) contamination/dilution of transport UW solution in 89 human pancreata processed by our islet isolation program. Samples of UW were taken from the primary pancreas container and examined for the presence of blood and or hemolysis. The cold ischemia time (CIT) was calculated, and processing by cold collagenase perfusion commenced. Islets were then isolated by collagenase digestion, purified by continuous gradient Ficoll centrifugation, and islet equivalent (IE) yield determined by dithizone staining. Viability of isolated islets was assayed by using the fluorescent SYTO/ethidium bromide (SYTO/EB) method. Na+ and K+ values were assayed using ion-specific electrode methodologies. Isolation outcomes were grouped by successful versus unsuccessful categories, and statistically compared using an unpaired t-test, where p < 0.05 was deemed to be statistically significant. Values are expressed as mean ± SEM. There was no significant relationship between increased Na+ and an unsuccessful isolation as a single variable. The study did suggest that the prolonged ischemic pancreas is more vulnerable to isolation failure if significant Na+ is present. In either case, a shorter CIT in UW was beneficial to increased IE yield and viability in human islet isolation.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".