Use of the Expanded Panel of<scp>BXD</scp>Mice Narrow<scp>QTL</scp>Regions in Ethanol‐Induced Locomotor Activation and Motor Incoordination
Bibliographic record
Abstract
BACKGROUND: Alcohol-related responses are under strong genetic regulation. A wealth of alcohol-related data from recombinant inbred (RI) mouse strains enables genetic correlation and mapping of these traits. Previous studies using RI strains have identified numerous chromosomal locations that underlie differential alcohol sensitivity, although the regions identified are typically large. One means to improve power and precision for genetic analysis is to use a larger genetic reference population. The expanded panel of BXD RI mice was used to identify quantitative trait loci (QTLs) associated with sensitivity to locomotor stimulatory and motor incoordinating effects of alcohol. The goals of this study were to determine whether previously reported QTLs were replicated and refined and to determine whether novel QTLs would be identified. METHODS: Following an i.p. dose of 2.25 g/kg of ethanol (EtOH) or saline control, locomotor activation was assessed using an activity chamber and motor incoordination was assessed using the accelerating rotarod. Male and female BXD mice from over 55 strains were tested. Two treatment paradigms were utilized to evaluate the effects of EtOH versus saline treatment-order. RESULTS: Activity chamber measures showed significant differences in strain, sex, and treatment-order whereas rotarod measures showed significant differences in strain and treatment-order. Significant QTLs for various measures of EtOH-induced locomotor activation were identified on chromosomes 2 and 5 that narrowed QTL regions previously identified from 19 to < 2 Mb. Further, a novel significant QTL for EtOH-induced motor incoordination on chromosome 7 was identified. CONCLUSIONS: Using the expanded RI BXD panel, along with a high precision marker map, several novel QTLs were found and several previously identified QTL regions were confirmed and narrowed. The isogenic nature of the population facilitated detection of treatment-order and sex-specific differences. Smaller QTL regions reduced the number of positional candidates thereby increasing the efficiency with which polymorphisms underlying the QTL will be identified.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.004 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".