Necrotic myositis of rainbow trout, <i>Oncorhynchus mykiss</i> (Walbaum): proteolytic characteristics of a crude extracellular preparation from <i>Flavobacterium psychrophilum</i>
Bibliographic record
Abstract
A crude extracellular preparation (CEP) from a strain of Flavobacterium psychrophilum recovered from a case of necrotic myositis affecting rainbow trout was capable of causing severe muscle necrosis in rainbow trout following intramuscular injection. Cell wall‐associated preparations, however, were unable to produce similar lesions in experimentally injected fish. The CEP degraded gelatin and type II collagen but not type I or type IV collagen. Furthermore, the CEP did not degrade 2‐furanacryloyl‐ l ‐leucylglycyl‐ l ‐prolyl‐alanine (FALGPA), chondroitin sulphates A, B or C, heparan sulphate, keratan sulphate, hyaluronic acid, elastin or rainbow trout erythrocytes. The addition of the protease inhibitors 1,10‐phenanthroline, ethylenediamine‐tetraacetic acid (EDTA) and EGTA to the CEP halted its ability to degrade gelatin in vitro and to produce muscle necrosis in rainbow trout in vivo . In vitro and in vivo activity was restored following the addition of 1 m m zinc chloride to the protease inhibitor‐treated CEP, suggesting that this strain of F . psychrophilum secretes a protein complex with zinc metalloprotease‐like activity. This protein complex, therefore, appears to be involved in the pathogenesis of necrotic myositis in rainbow trout.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".