Tinman is a direct activator of <i>midline</i> in the <i>drosophila</i> dorsal vessel
Bibliographic record
Abstract
Heart development requires a conserved core of transcription factors comprised of Nkx2.5, GATA and T-box family transcription factors. In Drosophila melanogaster, the Nkx2.5 gene tinman acts upstream of many cardiac genes including the Tbx20 homolog midline, a critical regulator of heart development in both flies and vertebrates. By testing genomic fragments containing clusters of consensus Tinman-binding sites, we identified a 4.3 kb fragment 5' of midline that directs reporter expression in all midline-expressing heart cells and a 1.7 kb subfragment that drives reporter expression in mid-expressing heart cells that maintain tin expression. Both fragments direct reporter gene expression in response to tinman in transgenic embryos and in transient transfection assays in Drosophila S2 cells. Mutation of two Tinman binding sites (Tin1 and Tin2) reduces or abolishes cardiac expression in derivatives of the 1.7 kb fragment. We conclude that Tin is a direct regulator of midline in fly heart development.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".