Alterations in fatty acid composition and fluidity of cell membranes affect the accumulation of PCB congener 2,2′,5,5′‐tetrachlorobiphenyl by <i>Ralstonia eutropha</i> H850
Bibliographic record
Abstract
Abstract The effect of alterations in fatty acid composition and fluidity of cell membranes on the accumulation of PCB congener 2,2′,5,5′‐tetrachlorobiphenyl (TeCB) by Ralstonia eutropha (formerly Alcaligenes eutrophus ) H850 was studied. Cells of R eutropha H850 grown on either biphenyl or fructose were used. Significant increases in saturated fatty acid composition and decreases in membrane fluidity of bacteria grown on biphenyl at 28 °C were observed compared with those grown on fructose at 17 or 28 °C. The ratio of saturated fatty acids to unsaturated fatty acids and membrane fluidity of R eutropha H850 grown on biphenyl at 28 °C resembled those of cells grown on fructose at 37 °C. No inhibition effect of the uncoupler 2,4‐dinitrophenol (2,4‐DNP) on TeCB accumulation was observed, suggesting an energy‐independent mechanism for TeCB accumulation in cells of R eutropha H850. The amount of TeCB accumulated was considerably higher in R eutropha H850 grown on fructose at 17 and 28 °C than when grown on biphenyl at 28 °C. Similar amounts of TeCB accumulated in bacteria grown on biphenyl at 28 °C as compared with those grown on fructose at 37 °C. These results suggest the alterations in fatty acid composition and membrane fluidity of R eutropha H850 may affect the accumulation of TeCB. © 2002 Society of Chemical Industry
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".