Rapid and Effective Germination Methods for Overcoming Seed Dormancy in Annual Canarygrass
Bibliographic record
Abstract
Dormancy in pedigreed Phalaris seed can lead to unsatisfactory germination in seed testing. The objective was to determine the effectiveness of recommended germination methods in overcoming seed dormancy in annual canarygrass (P. canariensis L.). In 2003, ‘CDC Maria’ was grown at two locations in Saskatchewan, Canada. At maturity, panicles were harvested and stored at –20°C. Four replications of 50 seeds were used in each experiment. Hulled and hand dehulled seed were (i) stored at 23°C for 0 to 16 wk before germinating at 23°C, (ii) germinated at 15 to 30°C (with and without light), (iii) allowed to imbibe water at 23°C for up to 96 h, (iv) treated with pricking, hydrogen peroxide (H2O2), gibberellic acid (GA), ethephon (2‐chloroethylphosphonic acid), potassium nitrate (KNO3), chilling, and heating before germinating at 23°C, and (v) treated with KNO3, heating, and chilling before germinating (with and without light) at 15°C, 15/25°C (16/8h), and 20/30°C (16/8h). Mean germination was tested to be significantly different (P = 0.05) from 95% germination by t tests. The after‐ripening period required to overcome primary dormancy in hulled seed was 8 to 10 wk longer than in dehulled seed. Hulled seed developed deeper secondary dormancy compared with dehulled seed, particularly at 20 and 25°C, with neither displaying <95% germination at 15°C or >10% at 30°C. Dehulled seed imbibed sufficient water to initiate germination 2 d faster than hulled seed. Applications of H2O2 and GA overcame dormancy in dehulled seed, while none of the treatments overcame hulled seed dormancy. Dehulled and hulled seed displayed unsatisfactory germination, regardless of treatment or light regime, when germinated under an alternating temperature regime of 20/30°C. We recommend (i) hand dehulling seed, (ii) treating hulled seed with 0.01M KNO3, or (iii) treating hulled seed with heat (3 d at 30°C) before germinating at 15°C for 10 d in darkness on top of blotters to overcome seed dormancy rapidly and effectively in annual canarygrass.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".