Effect of Seminal Plasma Protein on Postthaw Viability and Fertility of Arctic Char Spermatozoa
Bibliographic record
Abstract
Abstract Seminal plasma protein of Arctic char Salvelinus alpinus was characterized using sodium dodecyl sulfate (SDS) gel electrophoresis. Twelve protein bands with molecular weights of 7.2, 12.4, 15.3, 20.0, 20.4, 22.6, 39.4, 66.3, 74.0, 92.0, 94.5, and 130.1 kilodaltons (kDa) were detected. The effect of total seminal plasma protein and protein fractions of three categories (<50, 50–100, and >100 kDa) on postthaw sperm motility, viability, and fertility was tested. Incorporation of total seminal plasma protein, the fraction greater than 100 kDa, or the fraction less than 50 kDa into the semen extender (300 mmol of glucose/L of water, plus 10% methanol) had a deleterious effect on postthaw sperm motility, viability, and fertility in comparison with spermatozoa frozen in the semen extender only. However, adding the 50-100-kDa fraction of seminal plasma protein to the semen extender did not affect the postthaw sperm motility and fertility relative to spermatozoa frozen in the extender only. Further experiments are needed to test the effect of different concentrations of seminal plasma proteins alone or in a combination with other seminal plasma constituents on sperm physiology and viability during short-term storage and cryopreservation.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".