Production of Antibodies against Enterocin B for Immunological Detection and Purification Purposes
Bibliographic record
Abstract
Polyclonal antibodies were generated against the bacteriocin enterocin B by conjugating enterocin B (ENT) to bovine serum albumin (BSA) through a bifunctional crosslinking agent. Rabbits immunized with the ENT-BSA conjugates developed antibodies specific for enterocin B. Non-competive indirect (NCI) ELISAs were able to detect the presence of enterocin B in the supernatant of a producer strain and from a partially-purified sample. Competitive indirect (CI) ELISAs demonstrated concentrations of enterocin B ranging from 400-1600 ng ml−1 were required for 50% antibody binding inhibition. An immunoaffinity chromatography column was developed by linking the antibodies to CNBr-activated Sepahrose 4B. The purification method yielded pure enterocin; however, the harsh elution conditions did not render a regeneratable column.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".