Nodulation responses of the tropical perennial clover, <i>Trifolium semipilosum</i>, inoculated with compatible and incompatible microsymbionts
Bibliographic record
Abstract
The tropical perennial clover, T. semipilosum , and the species, T. repens , exhibited both similarities and differences in their nodulation characteristics following Rhizobium inoculation. Both T. semipilosum and T. repens were infected via root hairs, formed indeterminate nodules and commenced active nitrogen-fixation approximately 1 week after inoculation. Effective Rhizobium strains produced on average four nitrogen-fixing nodules per T. semipilosum plant, predominantly in the upper region of the tap root. In contrast, 65% of the average 15 nodules per T. repens plant were induced on lateral roots. The Rhizobium strain-ANU843, effective (Nod + Fix + ) on T. repens , caused root hair branching and twisting on T. semipilosum , although plants did not fix nitrogen, indicative of an ineffective host–strain interaction. Such ineffective interactions were associated with increased total numbers of nodule-like structures on T. semipilosum ; these nodules were atypically associated with, or in close proximity to, lateral roots. Using a GUS gene-modified rhizobia to monitor nodule occupancy, a third of the total nodule-like structures formed from the incompatible interactions showed a positive GUS reaction, indicating that colonization by Rhizobium strains of some of the nodule-like structures had occurred. Moreover, about 70% of the GUS positive nodule-like structures from the incompatible interactions were localized at the junction between the tap and lateral roots. These observations are consistent with an atypical infection route by the incompatible strains.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".