Expression of Cocoonase in Silkworm (Bombyx mori) Cells by Using a Recombinant Baculovirus and Its Bioactivity Assay
Bibliographic record
Abstract
In this research, the cocoonase gene was cloned by RT-PCR as an 860 bp fragment, including the signal peptide and thecore sequence of cocoonase gene. In order to investigate the function of signal peptide, recombinant transfer vectorpBacPAK8-Cocoonase-EGFP were constructed by fusing with enhanced green fluorescent protein (EGFP) gene toobserve under fluorescence microscope. The purified pBacPAK8-Cocoonase-EGFP DNA was co-transfected withlinear virus Bm-BacPAK6 DNA into BmN cells. The homologous recombination occurred in the cells and then therecombinant virus Bm-BacPAK8-Cocoonase-EGFP was obtained. BmN cell was infected with the recombinant virusBm-BacPAK8-Cocoonase-EGFP, and fluorescent signal was detected in most of the cells under fluorescencemicroscope at 72 hrs postinfection. Then BmN cells were harvested. Both SDS-PAGE and Western-blotting analysisindicated that the cocoonase was expressed successfully in silkworm (Bombyx mori) baculovirus expression vectorsystem. Furthermore, referred to Astrup methods,used fibrin plate process confirmed that expression product in vitrohad cellulolytic activity. We conclude that silkworm expression system can be used successfully to express functionalcocoonase.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".