Optical and Scanning Probe Analysis of Glycolipid Reorganization upon Concanavalin A Binding to Mannose-Coated Lipid Bilayers
Bibliographic record
Abstract
Molecular level associations between components within the lipid bilayer as proteins complex to the membrane surface are fundamental to the complete understanding of protein−membrane interactions. To characterize these protein-induced membrane-organizational processes, we have prepared a new fluorescently labeled glycolipid (PSMU) that enables monitoring of glycolipid aggregation within the lipid membrane. The glycolipid's mannosamine headgroup was specifically recognized by the lectin concanavalin A (Con A). Fluorescence studies with liposomes composed of 5 mol % PSMU/distearylphosphatidylcholine found that the membrane reorganized in response to Con A adsorption. Initially aggregated structures of glycolipid were dispersed as a consequence of specific affinity with the lectin through steric restrictions imposed by other bound Con A, the distance between mannosyl receptor sites, and possible protein insertion events. The protein binding and membrane reorganizational process was slow (ca. days). An association constant for Con A with the glycolipid membrane was estimated to be 3 × 10 6 M - 1, around 2 orders of magnitude higher than that for methyl- d -α-mannopyranoside. Nanoscale imaging with the atomic force microscope found that the glycolipid formed 10 nm wide dendrite structures throughout the membrane and that the bound Con A was associated with those nanoscale features.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".