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Record W2008060163 · doi:10.2135/cropsci2008.03.0145

Tagging and Mapping <i>Pse‐1</i> Gene for Resistance to Halo Blight in Common Bean Differential Cultivar UI‐3

2009· article· en· W2008060163 on OpenAlexaboutno aff
Phillip N. Miklas, Deidré Fourie, Jennifer K. Wagner, R. C. Larsen, Charlotte Mienie

Bibliographic record

VenueCrop Science · 2009
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicPlant pathogens and resistance mechanisms
Canadian institutionsnot available
Fundersnot available
KeywordsBiologyBulked segregant analysisGermplasmRAPDCultivarPopulationGeneticsPhaseolusPseudomonas syringaeQuantitative trait locusPlant disease resistanceMicrosatelliteGenetic markerMarker-assisted selectionGeneBotanyGene mappingAlleleGenetic diversityChromosome

Abstract

fetched live from OpenAlex

ABSTRACT Halo blight [caused by Pseudomonas syringae pv. phaseolicola (Burkh.) Young et al. ( Psp )] is a serious seed‐borne bacterial disease of common bean ( Phaseolus vulgaris L.). A few resistance (R) genes and quantitative trait loci provide control to one or more races of the pathogen. To better understand monogenic resistance and improve breeding efficiency, we sought to tag and map a gene ( Pse‐1 ) in host differential cultivar UI‐3 (previously named ‘Red Mexican UI‐3’) that provides resistance to races 1, 5, 7, and 9 of Psp Cosegregation for resistance to races 1, 5, 7, and 9, in a recombinant inbred population, ‘Canadian Wonder’/UI‐3 (CU), confirmed the effect of Pse‐1 against multiple races of the pathogen. Bulked‐segregant analysis in the CU population identified six random amplified polymorphic DNA (RAPD) markers tightly linked (0–3.3 cM) to Pse‐1 . Three of the RAPDs completely linked with Pse‐1 in the CU population were converted to sequence characterized amplified region (SCAR) markers SH11.800, SR13.1150, and ST8.1350. The linked markers were used to integrate Pse‐1 to linkage group B10 of the core map. Allelism tests (F 2 ) confirmed relationships of Pse‐1 and Pse‐4 derived from UI‐3 with R genes in the other host differential cultivars. A survey of advanced lines and cultivars revealed that the SCAR markers generated in this study will have utility for marker‐assisted selection of Pse‐1 in germplasm from the Andean gene pool (e.g., kidney, calima) and from race Mesoamerican within the Middle American gene pool (black, carioca).

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.342
Threshold uncertainty score0.418

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0010.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.017
GPT teacher head0.227
Teacher spread0.209 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations32
Published2009
Admission routes1
Has abstractyes

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