Ethanol Inhibits Gap‐Junctional Coupling Between P19 Cells
Bibliographic record
Abstract
BACKGROUND: Gap junctions are plaques of multiple intercellular channels that connect the cytoplasm of adjacent cells. They provide both electrical and metabolic coupling and are an essential element in normal growth, development, and physiology. Little research exists on the relationship between alcohol administration and gap-junctional function or expression. This study looks at the function and expression of gap junctions after incubation and withdrawal of ethanol with P19 cell cultures. METHODS: Gap-junctional communication was assessed after 24 and 48 hr of exposure to 20 and 40 mM ethanol and after a 24-hr withdrawal period. The seeding technique was used, and diacyl-3,3'-indocarbocyanine iodide/calcein-stained donor cells were seeded on an unstained monolayer and then reviewed by confocal microscope and counted by flow cytometry. Analysis of connexin (Cx) proteins was performed by Western blot, gel electrophoresis, and immunoblots with antibodies for Cx26 and Cx43. RESULTS: All treatment regimens produced similar results, reducing dye coupling by more than 50% without recovery after a 24-hr withdrawal period. Exposing the cells to 20 mM ethanol for 48 hr did not significantly change the levels of Cx26 protein, but ethanol significantly decreased the levels of Cx43 in cultured P19 cells. CONCLUSIONS: This study illustrates that ethanol can inhibit gap-junction function in the P19 cell line. Chronic exposure to 20 mM ethanol selectively decreased the levels of Cx43 protein in the membrane fraction of the cell cultures.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".