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Abstract LB-168: A hexane fraction of American ginseng suppresses colon cancer associated with colitis

2012· article· en· W2009233027 on OpenAlexaff
Deepak Poudyal, Xiangli Cui, Anne B. Hofseth, Alena Chumanevich, Alexander A. Chumanevich, Michael J. Wargovich, Anthony Windust, Mitzi Nagarkatti, Prakash Nagarkatti, Lorne J. Hofseth

Bibliographic record

VenueCancer Research · 2012
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGinseng Biological Effects and Applications
Canadian institutionsNational Research Council Canada
Fundersnot available
KeywordsAzoxymethaneColorectal cancerCancerApoptosisMedicineColitisInflammationCancer researchCarcinogenesisGastroenterologyInternal medicineChemistryBiochemistry

Abstract

fetched live from OpenAlex

Abstract Patients with Ulcerative Colitis (UC) have a high risk of colon cancer. We have recently shown American Ginseng (AG) suppresses colitis, and prevents colon cancer in mice. In the present study we isolated a Hexane Fraction of AG and found this to have particularly potent anti-oxidant and pro-apoptotic properties. The Hexane Fraction of AG was effective in inducing apoptosis of human epithelial colon cancer cell, HCT-116 and inflammatory cells. As well, this fraction was shown to suppress azoxymethane (AOM)/DSS-induced colon cancer. We performed two studies with mice: a short term (Day 35) and long term (Day 50) cancer prevention study. Mice fed the Hexane fraction of AG had significantly less inflammatory and ulcerative lesions, as well as cancerous lesions compared with the vehicle-treated group in both the short and long-term cancer prevention studies. Results are consistent with the hypothesis that the Hexane fraction of AG has anti-inflammatory and anti-cancer properties, and prevents the progression of colon cancer in AOM/DSS mouse model. Interestingly, at Day 35, following isolation of CD45- epithelial cells from colons of mice treated with either AOM+DSS+Vehicle or AOM+DSS+Hexane fraction of AG, there was a 24% increase in the putatative tumor suppressor miRNA-16 with treatment with the Hexane fraction of AG. When looking at putative miRNAs with oncogenic properties (miRNA-21 and -155), levels were decreased in epithelial cells by 25% and 12% respectively in the Hexane fraction of AG-treated mice. This study provides additional pre-clincal data, and mechanistic studies that support the possible use of this complementary and alternative medicine in the treatment of colitis, and the prevention of colon cancer associated with colitis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-168. doi:1538-7445.AM2012-LB-168

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.434
Threshold uncertainty score0.533

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.052
GPT teacher head0.404
Teacher spread0.351 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations0
Published2012
Admission routes1
Has abstractyes

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