Interaction of dopamine D1 receptor with N‐ethylmaleimide‐sensitive factor is important for the membrane localization of the receptor
Bibliographic record
Abstract
The dopamine D1 receptor (D1R) plays important roles in regulating motor coordination, working memory, learning, and reward. In the mammalian brain, D1R is localized predominantly in dendritic spines. However, the molecular mechanisms involved in the transport, sorting, and targeting of D1R to dendritic spines are largely unknown. Here, we characterize the interaction between D1R and N-ethylmaleimide-sensitive factor (NSF) and show that the interaction is mediated by aa 387-401 of the D1R C-terminal tail. Interfering D1R and NSF interaction by coexpressing GFP-D1R aa 387-401 fusion protein reduces D1R membrane localization and inhibits D1R mediated cAMP accumulation. Treatment of hippocampal neurons with Tat-D1R aa 387-401 decreases the synaptic localization of D1R and the cell surface expression of D1R, but not the cell surface expression of alpha7 nicotinic receptor. Our data indicate that the interaction between NSF and D1R is important for the membrane localization of D1R.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".